Gel Permeation Chromatography (GPC) is a separation method for the determination of molecular weight averages (Mn) and molecular weight distribution (PDI=Mw/Mn). GPC separates molecules on the basis of hydrodynamic size rather than molecular weight. The large molecules move more rapidly through the GPC column, and in this way the mixture can be separated.
In addition to the built-in refractive index and UV detectors, this GPC is also equipped with a Wyatt Technologies TREOS three-angle light-scattering detector (useful for absolute molecular weight determination) and a Wyatt ViscoStar detector (measures specific viscosity, which can be used to derive the intrinsic viscosity). Intrinsic viscosity is best utilized together with molar mass information from light scattering to derive the hydrodynamic radius, conformation, and branching ratio of a macromolecule in solution.
- Solvent: THF
- MW range: 500-1,000,000 Da
- Suitable for polymerization mixtures, block and random copolymers
- Calibration standard: polystyrene (PS)
- Equipped with quadruple-detection capability: differential refractive index (RI) detector, UV detector, three-angle light-scattering detector, and viscometer